Apparatus for continuously referenced analysis of reactive components in solution

Patent · Thu Jan 01 00:00:00 EST 1981

OSTI ID:863866

Bostick, William D ^[1]; Denton, Mark S ^[1]; Dinsmore, Stanley R ^[2]

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1. Oak Ridge, TN
2. Norris, TN

A continuously referenced apparatus for measuring the concentration of a reactive chemical species in solution comprises in combination conduit means for introducing a sample solution, means for introducing one or more reactants into a sample solution, a reaction zone in fluid communication with said conduit means wherein a first chemical reaction occurs between said species and reactants, and a stream separator disposed within the conduit means for separating the sample solution into a sample stream and a reference stream. An enzymatic reactor is disposed in fluid communication with only the sample stream wherein a second reaction takes place between the said reactants, species, and reactor enzymes causing the consumption or production of an indicator species in just the sample stream. Measurement means such as a photometric system are disposed in communication with the sample and reference streams, and the outputs of the measurement means are compared to provide a blanked measurement of the concentration of indicator species. A peristaltic pump is provided to equalize flow through the apparatus by evacuation. The apparatus is particularly suitable for measurement of isoenzymes in body tissues or fluids.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4263406
• OSTI ID: 863866
• Country of Publication: United States
• Language: English

Related Subjects

apparatus
continuously
referenced
analysis
reactive
components
solution
measuring
concentration
chemical
species
comprises
combination
conduit
means
introducing
sample
reactants
reaction
zone
fluid
communication
occurs
stream
separator
disposed
separating
reference
enzymatic
reactor
takes
enzymes
causing
consumption
production
indicator
measurement
photometric
streams
outputs
compared
provide
blanked
peristaltic
pump
provided
equalize
flow
evacuation
particularly
suitable
isoenzymes
tissues
fluids
reactive chemical
sample stream
reaction takes
solution comprises
sample solution
particularly suitable
reaction zone
chemical species
chemical reaction
fluid communication
conduit means
reaction occurs
chemical specie
peristaltic pump
active components
reactive components
active chemical
active component
reaction occur
continuously referenced
/435/422/