Genomic organization and sequence of the rat major histocompatibility complex class Ia gene RT1.A{sup u}
- Abteilung Immungenetik der Universitaet, Goettingen (Germany)
From a rat cosmid library of LEW.1W genomic DNA (RT1{sup u}) in the pWE15 vector cosmid clone 9b1 was isolated by using rat MHC probe LW2. Clone 9b1 was mapped to the RT1.A region by Southern blotting of DNA of RT1 recombinant congenic rat strains. After stable transfection of cosmid 9b1 into mouse L cells and flow cytometry analysis, a high level of expression (about 100-fold compared with controls) was observed with RT1.A{sup u} class I antigen-specific rat monoclonal antibody NR3/31. Thus cosmid 9b1 contains a complete functional class I gene. Since this gene was derived from the RT1.A region and showed a class Ia-like expression and since its gene product reacted with an RT1.A{sup u} antigen-specific antibody, the clone described here must represent a class Ia RT1.A{sup u} gene. 8 refs., 2 figs.
- OSTI ID:
- 75568
- Journal Information:
- Immunogenetics, Vol. 41, Issue 5; Other Information: PBD: Mar 1995
- Country of Publication:
- United States
- Language:
- English
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