Effect of genes controlling radiation sensitivity on chemically induced mutations in Saccharomyces cerevisiae. [UV radiation]
The effect of 16 different genes (rad) conferring radiation sensitivity on chemically induced reversion in the yeast Saccharomyces cerevisiae was determined. The site of reversion used was a well-defined chain initiation mutant mapping in the structural gene coding for iso-1-cytochrome c. High doses of EMS and HNO/sub 2/ resulted in decreased reversion of cyc1-131 in rad6, rad9 and rad15 strains compared to the normal RAD + strains. In addition, rad52 greatly decreased EMS reversion of cyc1-131 but had no effect on HNO/sub 2/-induced reversion; rad18, on the other hand, increased HNO/sub 2/-induced reversion but did not alter EMS-induced reversion. When NQO was used as the mutagen, every rad gene tested, except for rad18, had an effect on reversion; rad6, rad9, rad15, rad17, rad18, rad22, rev1, rev2, and rev3 lowered NQO reversion while rad1, rad2, rad3, rad4, rad10, rad12, and rad16 increased it compared to the RAD + strain. The effect of rad genes on chemical mutagenesis is discussed in terms of their effect on uv mutagenesis. It is concluded that although the nature of the repair pathways may differ for uv- and chemically-induced mutations in yeast, a functional repair system is required for the induction of mutation by the chemical agents NQO, EMS, and HNO/sub 2/.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 7347523
- Journal Information:
- Genetics; (United States), Vol. 83:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EMS
BIOLOGICAL EFFECTS
GENES
NITROUS ACID
ORGANIC NITROGEN COMPOUNDS
QUINOLINES
SACCHAROMYCES CEREVISIAE
MUTATIONS
RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
MUTAGENESIS
ULTRAVIOLET RADIATION
AZINES
BIOMASS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
ESTERS
FUNGI
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INORGANIC ACIDS
MICROORGANISMS
MUTAGENS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PYRIDINES
RADIATION EFFECTS
RADIATIONS
RENEWABLE ENERGY SOURCES
SACCHAROMYCES
SULFONIC ACID ESTERS
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)