Ultrastructural and physico-chemical characterization of chloroplasts isolated from Euglena gracilis var. bacillaris at selected developmental stages

Technical Report · Mon Dec 01 00:00:00 EST 1975

OSTI ID:7326438

Palisano, J R

Plastids at selected stages of development were isolated from the unicellular green alga, Euglena gracilis Klebs var. bacillaris Pringsheim, and were characterized by physico-chemical techniques. This investigation included the devising of a single isosmotic gradient in which plastids at each of the developmental stages selected could be isolated; the determination of the sedimentation coefficients of plastids at selected stages of development; the characterization of the structure of the isolated plastids, using electron and light microscopy, and where possible, the correlation of structure with sedimentation coefficients and enzymatic activity of the isolated plastids. The methods employed in this study included electron microscopy, bright-field and fluorescence microscopy, zonal ultracentrifugation, computer programming, protein determination, and enzymatic analyses.

• Research Organization: Tennessee Univ., Knoxville (USA)
• DOE Contract Number: E(40-1)-GEN-33
• OSTI ID: 7326438
• Report Number(s): TID-27217
• Resource Relation: Other Information: Thesis
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLOROPLASTS
CHEMICAL PROPERTIES
PHYSICAL PROPERTIES
EUGLENA
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
COMPUTER CALCULATIONS
ELECTRON MICROSCOPY
ENZYMES
MEASURING METHODS
MICROSCOPY
PROTEINS
SEDIMENTATION
ALGAE
BIOMASS
ENERGY SOURCES
KINETICS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
RENEWABLE ENERGY SOURCES
UNICELLULAR ALGAE
550200* - Biochemistry