Carbohydrates of influenza virus. I. Glycopeptides derived from viral glycoproteins after labeling with radioactive sugars. [/sup 14/C, /sup 3/H]

Journal Article · Mon Aug 01 00:00:00 EDT 1977 · J. Virol.; (United States)

OSTI ID:7312212

Schwarz, R T; Schmidt, M F.G.; Anwer, U; Klenk, H D

The carbohydrate moiety of the influenza glycoproteins NA, HA/sub 1/, and HA/sub 2/ were analyzed by labeling with radioactive sugars. Analysis of glycopeptides obtained after digestion with Pronase indicated that there are at least two different types of carbohydrate side chains. The side chain of type I is composed of glucosamine, mannose, galactose, and fucose. It is found on NA, HA/sub 1/, and HA/sub 2/. The side chain of type II contains a high amount of mannose and is found only on NA and HA/sub 2/. The molecular weights of the corresponding glycopeptides obtained from virus grown in chicken ambryo cells are 2,600 for type I and 2,000 for type II. The glycoproteins of virus grown in MDBK cells have a higher molecular weight than those of virus grown in chicken embryo cells, and there is a corresponding difference in the molecular weights of the glycopeptides. Under conditions of partial inhibition of glycosylation, virus particles were isolated that contained hemagglutinin with reduced carbohydrate content. Glycopeptide analysis indicated that this reduction is due to the lack of whole carbohydrate side chains and not to the incorporation of incomplete ones. This observation suggests that glycosylation of the viral glycoproteins involves en bloc transfer of the core sugars to the polypeptide chains.

• Research Organization: Univ., Giessen, Germany
• OSTI ID: 7312212
• Journal Information: J. Virol.; (United States), Vol. 23:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBOHYDRATES
BIOCHEMICAL REACTION KINETICS
RADIONUCLIDE KINETICS
GLUCOPROTEINS
INFLUENZA VIRUSES
SACCHARIDES
CARBON 14
LABELLING
MOLECULAR WEIGHT
TRITIUM
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
EVEN-EVEN NUCLEI
HYDROGEN ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VIRUSES
YEARS LIVING RADIOISOTOPES
550200* - Biochemistry
560174 - Radiation Effects- Nuclide Kinetics & Toxicology- Microorganisms- (-1987)