Soluble methane monooxygenase component B gene probe for identification of methanotrophs that rapidly degrade trichloroethylene

Journal Article · Sun Mar 01 00:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7278594

Tsien, Hsienchyang; Hanson, R S ^[1]

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1. Univ. of Minnesota, Navarre (United States)

Restriction fragment length polymorphisms, Western blot (immunoblot) analysis, and fluorescence-labelled signature probes were used for the characterization of methanotrophic bacteria as well as for the identification of methanotrophs which contained the soluble methane monooxygenase (MMO) gene and were able to degrade trichloroethylene (TCE). The gene encoding a soluble MMO component B protein from Methylosinus trichosporium OB3b was cloned. It contained a 2.2-kb EcoRI fragment. With this cloned component B gene as probe, methanotroph types I, II, and X and environmental and bioreactor samples were screened for the presence of the gene encoding soluble MMO. Among twelve pure or mixed cultures, DNA fragments of seven methanotrophs hybridized with the soluble MMO B gene probe. When grown in media with limited copper, all of these bacteria degraded TCE. All of them are type II methanotrophs. The soluble MMO component B gene of the type X methanotroph, Methylococcus capsulatus Bath, did not hybridize to the M. trichosporium OB3b soluble MMO component B gene probe, although M. capsulatus Baath also produces a soluble MMO.

• OSTI ID: 7278594
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 58:3; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLORINATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
OXYGENASES
GENES
BIOMIMETIC PROCESSES
COPPER
DETECTION
DNA-CLONING
METABOLISM
METHANE
METHANOTROPHIC BACTERIA
ORGANIC CHLORINE COMPOUNDS
RFLPS
SOLVENTS
ALKANES
BACTERIA
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA HYBRIDIZATION
ELEMENTS
ENZYMES
HALOGENATED ALIPHATIC HYDROCARBONS
HYBRIDIZATION
HYDROCARBONS
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PROTEINS
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology