Production and analysis of the biopolymer chitosan from Mucor rouxii. Final report, December 1985-July 1987
To determine the potential for the production of chitosan with physiochemical properties from a fungal source, growth studies were conducted using Mucor rouxii. Growth of the organism under a variety of conditions was studied to determine the effects on polymer molecular-weight distributions and biomass production. Modifications of processing protocols were also evaluated to examine the effects of yields of chitosan extracted from the fungal cell wall as well as molecular-weight distribution. This represents the first such study where these factors were correlated to the yield and molecular-weight distribution of chitosan. Of the growth parameters evaluated, length of incubation, culture volume, source of inorganic salt in defined medium, and medium component concentration in complex medium had an effect on biomass and MW distributions. Processing parameters affecting the amount of chitosan extracted were the type and strength of acid and the homogenization of cell wall material prior to refluxing. Overall, weight average molecular weights of chitosan varied up to 8-fold in studies relating to fungal age, while up to 2-fold changes in molecular weight were affected by pH, medium type, and culture vessel size.
- Research Organization:
- Army Natick Research and Development Center, MA (USA)
- OSTI ID:
- 7271717
- Report Number(s):
- AD-A-214111/7/XAB; NATICK/TR-88/046
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL MATERIALS
POLYMERS
BIOMASS
PRODUCTION
CHITIN
BIOCONVERSION
BIOCHEMISTRY
CELL WALL
CULTURE MEDIA
DISTRIBUTION
FERMENTATION
FUNGI
INCUBATION
LENGTH
MODIFICATIONS
MOLECULAR WEIGHT
NUTRIENTS
POLYSACCHARIDES
PROCESSING
PROGRESS REPORT
SALTS
VOLUME
AMINES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMISTRY
DIMENSIONS
DOCUMENT TYPES
ENERGY SOURCES
MATERIALS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
RENEWABLE ENERGY SOURCES
SACCHARIDES
090800* - Biomass Fuels- Production- (1990-)
091000 - Biomass Fuels- Properties & Composition- (1990-)