Role of x-ray-induced transcripts in adaptive responses following x-rays
Potentially lethal damage repair (PLDR) and sublethal damage repair, may be the same manifestations of a series of common enzymatic steps. PLDR, has two distinct phases based upon DNA lesion repair and survival recovery studies. The first phase of PLDR occurs very quickly (t{sub {1/2}}:2--20 mins) to increase the survival of X-irradiated cells by mending the vast array of DNA lesions created by ionizing radiation. The second slower phase of PLDR proceeds much later (i.e., >1--2 hrs) following X-irradiation, during which the remaining double-stranded DNA breaks are completely repaired. This second phase of repair closely corresponds to the restructuring of gross chromosomal damage, and can be partially blocked in some human cells by inhibiting protein synthesis. This slower phase of PLDR correlated with a rapid decline in X-ray-induced transformation of normal cells. The fast component of PLDR may be due to constitutively synthesized DNA ligases, topoisomerases, or polymerases, which act immediately to repair damaged, DNA. In contrast, the slow phase of PLDR in human cells may require the induction of specific genes and gene product's involved in the repair of potentially lethal or carcinogenic DNA lesions. Induced gene products (i.e., proteins) specifically synthesized in response to physiological doses of ionizing radiation in radioresistant human melanoma (U1-Mel) cells, and in a variety of other human normal and cancer-prone cells, were identified using two-dimensional gel electrophoresis. We identified and partially characterized ten proteins synthesized by U1-Mel cells. The synthesis of eight of these proteins were specifically induced by ionizing radiation and two proteins were repressed Neither heat shock, UV-irradiation, nor bifunctional alkylating agent treatments resulted in the induction of these proteins. The expression of one protein, XIP269, correlated very well with PLDR capacity.
- Research Organization:
- Univ. of Michigan, Ann Arbor, MI (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER61256
- OSTI ID:
- 7249666
- Report Number(s):
- DOE/ER/61256-1; ON: DE92040814
- Country of Publication:
- United States
- Language:
- English
Similar Records
Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation
Role of x-ray-induced transcripts in adaptive responses following x-rays. Progress report, Year 2
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA HELICASES
ENZYME INDUCTION
DNA POLYMERASES
DNA REPAIR
MOLECULAR BIOLOGY
ENDONUCLEASES
RADIOBIOLOGY
BIOLOGICAL PATHWAYS
AUTORADIOGRAPHY
BIOLOGICAL ADAPTATION
DNA-CLONING
PROGRESS REPORT
STRAND BREAKS
THYMIDINE
TRITIUM COMPOUNDS
TWO-DIMENSIONAL ELECTROPHORESIS
X RADIATION
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CLONING
DNA HYBRIDIZATION
DNA-ASE
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
ESTERASES
GENE REGULATION
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROLASES
IONIZING RADIATIONS
NUCLEOSIDES
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
PYRIMIDINES
RADIATIONS
REPAIR
RIBOSIDES
TRANSFERASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques