Extracellular calcium alters the effects of retinoic acid on DNA synthesis in cultured murine keratinocytes
The rate of proliferation of epidermal keratinocytes was manipulated by growing the cells in medium containing high or low concentrations of calcium. Keratinocytes cultured in high extracellular Ca/sup + +/ (1.4 mM and 2.8 mM) proliferated twice as fast as those grown in low Ca/sup + +/ medium (0.09 mM) as measured by incorporation of (/sup 3/H) thymidine into DNA. Exposure of high calcium keratinocytes to all-trans retinoic acid for 4 days caused a dose-related inhibition of DNA synthesis with an IC/sub 50/ of about 10 ..mu..M. In contrast, incubating low calcium keratinocytes with all-trans retinoic acid caused a dose-related stimulation of DNA synthesis with maximum increase of 278% over control at 10 ..mu..M. This increase was accompanied by increases in culture confluency with maximum increase of 109% in cell number of control at 10 ..mu..M. These results are of importance since they suggest Ca/sup + +/ may influence the effect of retinoids on keratinocytes.
- Research Organization:
- Herbert Labs., Irvine, CA
- OSTI ID:
- 7245156
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 138:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CALCIUM COMPOUNDS
BIOLOGICAL EFFECTS
DNA
BIOSYNTHESIS
EPIDERMIS
CELL PROLIFERATION
RETINOIC ACID
CATIONS
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
INHIBITION
MICE
RESPONSE MODIFYING FACTORS
STIMULATION
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CARBOXYLIC ACID ESTERS
CHARGED PARTICLES
DATA
EPITHELIUM
ESTERS
HETEROCYCLIC COMPOUNDS
INFORMATION
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
RODENTS
SKIN
SYNTHESIS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology