Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Journal of Immunology; (USA)

OSTI ID:7244336

Muto, M; Kubo, E; Kamisaku, H; Sado, T ^[1]

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1. National Institute of Radiological Sciences, Chiba (Japan)

Using an intrathymic injection assay on B10 Thy-1 congenic mice, it was demonstrated that thymic prelymphoma cells first developed within the thymuses from 4 to 8 days after split-dose irradiation and were detected in more than 63% of the test donor thymuses when examined at 21 and 31 days after irradiation. Moreover, some mice (25%) at 2 mo after split-dose irradiation had already developed thymic lymphomas in their thymuses. To characterize these thymic prelymphoma cells, the thymocytes from B10 Thy-1.1 mice 1 mo after irradiation were stained with anti-CD4 and anti-CD8 mAb and were sorted into four subpopulations. These fractionated cells were injected into the recipient thymuses to examine which subpopulation contained thymic prelymphoma cells. The results indicated that thymic prelymphoma cells existed mainly in CD4- CD8- and CD4- CD8+ thymocyte subpopulations and also in CD4+ CD8+ subpopulation. T cell lymphomas derived from CD4- CD8- prelymphoma cells had mainly CD4- CD8- or CD4- CD8+ phenotypes. T cell lymphomas developed from CD4- CD8+ prelymphoma cells mainly expressed CD4- CD8+ or CD4+ CD8+ phenotype. T cell lymphomas originating from CD4+ CD8+ prelymphoma cells were mainly CD4+ CD8+ but some CD4- CD8+ or CD4+ CD8- cells were also present. These thymic prelymphoma cells were further characterized phenotypically in relation to their expression of the marker defined by the mAb against J11d marker and TL-2 (thymus-leukemia) Ag, which is not expressed on normal thymocytes of B10.Thy-1.2 or B10.Thy-1.1 strain, but appears on the thymocytes of lymphomagenic irradiated mice. The results indicated that the prelymphoma cells existed in J11d+, TL-2+ cells.

• OSTI ID: 7244336
• Journal Information: Journal of Immunology; (USA), Vol. 144:3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOMAS
RADIOINDUCTION
THYMUS
THYMUS CELLS
BIOLOGICAL RADIATION EFFECTS
ANTIGENS
BIOLOGICAL MARKERS
FRACTIONATED IRRADIATION
LYMPHOCYTES
MICE
MONOCLONAL ANTIBODIES
PHENOTYPE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
IMMUNE SYSTEM DISEASES
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
NEOPLASMS
ORGANS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals