Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1
Journal Article
·
· Applied and Environmental Microbiology; (United States)
OSTI ID:7224317
- Univ. of Iowa, Iowa City (United States)
Escheria coli JM109(pDTG601), containing the todC1C2BA genes encoding toluene dioxygenase from Pseudomonas putida F1, oxides indan to (-)-(1R)-indanol (83{percent} R) and trans-1,3-indandiol. Under similar conditions, P.putida F39/D oxidizes indan to (-)-(1R)-indanol (96{percent}R), 1-indanone, and trans-1,3-indandiol. The differences in the enantiomeric composition of the 1-indanols formed by the two organisms are due to the presence of a 1-indanol dehydrogenase in P.putida F39/D that preferentially oxidizes (+)-(1S)-indanol.
- OSTI ID:
- 7224317
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 58:10; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Fri Dec 01 00:00:00 EST 1995
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OSTI ID:7224317
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ESCHERICHIA COLI
GENETIC ENGINEERING
INDAN
HYDROXYLATION
OXYGENASES
DNA-CLONING
TOLUENE
BIODEGRADATION
PSEUDOMONAS
RECOMBINANT DNA
STEREOCHEMISTRY
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOTECHNOLOGY
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA
DNA HYBRIDIZATION
ENZYMES
HYBRIDIZATION
HYDROCARBONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
550400* - Genetics
560300 - Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ESCHERICHIA COLI
GENETIC ENGINEERING
INDAN
HYDROXYLATION
OXYGENASES
DNA-CLONING
TOLUENE
BIODEGRADATION
PSEUDOMONAS
RECOMBINANT DNA
STEREOCHEMISTRY
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOTECHNOLOGY
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA
DNA HYBRIDIZATION
ENZYMES
HYBRIDIZATION
HYDROCARBONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
550400* - Genetics
560300 - Chemicals Metabolism & Toxicology