Degradation of 1,2-dichloroethane by Ancylobacter aquaticus and other facultative methylotrophs
- Univ. of Groningen (Netherlands)
Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to row on 2-chloroethanol, chloroacetate, and 2-chloropropionate. In all strains tested, DCE was degraded by initial hydrolytic dehalogenation to 2-chloroethanol, followed by oxidation by a phenazine methosulfate-dependent alcohol dehydrogenase and an NAD-dependent aldehyde dehydrogenase. The resulting chloroacetic acid was converted to glycolate by chloroacetate dehalogenase. The alcohol dehydrogenase was induced during growth on methanol or DCE in strain AD20, but no activity was found during growth on glucose. However, in strain AD25 the enzyme was synthesized to a higher level during growth on glucose than on methanol, and it reached levels of around 2 U/mg of protein in late-exponential-phase cultures growing on glucose. The haloalkane dehalogenase was constitutively produced in all strains tested, but strain AD25 synthesized the enzyme at a level of 30 to 40% of the total cellular protein, which is much higher than that found in other DCE degraders. The nucleotide sequences of the haloalkane dehalogenase (dhlA) genes of strains AD20 and AD25 were the same as the sequence of dhlA from Xanthobacter autotrophicus GJ10 and GJ11. Hybridization experiments showed that the dhlA genes of six different DCE utilizers were all locate on an 8.3-kb EcoRI restriction fragment, indicating that the organisms may have obtained the dhlA gene by horizontal gene transmission.
- OSTI ID:
- 7206272
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 58:3; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLORINATED ALIPHATIC HYDROCARBONS
BIODEGRADATION
ALCOHOL DEHYDROGENASE
BACTERIA
DNA HYBRIDIZATION
DNA SEQUENCING
GENETIC MAPPING
GLUCOSE
METHANOL
MOLECULAR BIOLOGY
SOLVENTS
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
HALOGENATED ALIPHATIC HYDROCARBONS
HEMIACETAL DEHYDROGENASES
HEXOSES
HYBRIDIZATION
HYDROXY COMPOUNDS
MAPPING
MICROORGANISMS
MONOSACCHARIDES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PROTEINS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
560300* - Chemicals Metabolism & Toxicology