Insulin and insulin-like growth factors stimulate deoxyribonucleic acid synthesis in PC12 pheochromocytoma cells
The effects of insulin and insulin-like growth factors (IGFs) on the replication of PC12 pheochromocytoma cells were investigated. Incubation of PC12 cells for 2-3 days in low (0.3%) serum medium decreased (/sup 3/H)thymidine incorporation into PC12 cell DNA to approximately 30% of that in control (15% serum) medium. Incubation of the cells in low serum medium also slowed the growth of the cultures and increased the percentage of cells in the G0/G1 phase of the cell cycle. Addition of insulin to cells in low serum medium increased (/sup 3/H)thymidine incorporation into the cells, increased the number of cells in PC12 cultures, and decreased the percentage of cells in the G0/G1 phase of the cell cycle. IGF-I and IGF-II also increased (/sup 3/H)thymidine incorporation into PC12 cells incubated in low serum medium. IGF-I (EC50, approximately 0.3 nM) was a more potent stimulus of (/sup 3/H)thymidine incorporation than was insulin (EC50, approximately 3.5 nM). These data suggest that insulin and IGFs are growth factors for PC12 cells, and that the growth-promoting effects of these agents may be mediated by a type I IGF receptor on PC12 cells.
- Research Organization:
- Univ. of Chicago, IL (USA)
- OSTI ID:
- 7196260
- Journal Information:
- Endocrinology; (United States), Vol. 122:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of insulin-like growth factor I and insulin receptors on cultured bovine adrenal fasciculata cells. Role of these peptides on adrenal cell function
Insulin-like growth factor I has independent effects on bone matrix formation and cell replication
Related Subjects
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
INSULIN
TUMOR CELLS
DNA REPLICATION
CELL CULTURES
CELL CYCLE
RATS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
HETEROCYCLIC COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MITOGENS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques