Dose-response relationship for ultraviolet-light-induced mutations at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster ovary cells

Journal Article · Wed Jan 01 00:00:00 EST 1975 · Somatic Cell Genet.; (United States)

DOI:https://doi.org/10.1007/BF01538669· OSTI ID:7187479

Hsie, A W ^[1]; Brimer, P A; Mitchell, T J; Gosslee, D G

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1. Oak Ridge National Lab., TN

Exposure of Chinese hamster ovary (CHO) cells clone K/sub 1/BH/sub 4/ to ultraviolet (uv) light at doses up to 86 ergs/mm/sup 2/ did not significantly reduce cell survival, but uv doses of 86-648 ergs/mm/sup 2/ produced an exponential cell killing. Observed mutation frequency to 6-thioguanine resistance induced by uv increases approximately in proportion to increasing doses up to 260 ergs/mm/sup 2/ in a range of 5-648 ergs/mm/sup 2/ examined. The pooled data of mutation frequency f(X) as a function of dose X from 0-260 ergs/mm/sup 2/ is adequately described by f(X) = 10/sup -6/ (13.6 + 2.04 X). That the uv-induced mutations to 6-thioguanine resistance affects the hyposanthine-guanine phosphoribosyltransferase (HGPRT) locus is supported by the observation that all randomly isolated drug-resistant colonies contained highly reduced or undetectable HGPRT activity.

• OSTI ID: 7187479
• Journal Information: Somatic Cell Genet.; (United States), Vol. 1:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
MUTATIONS
CELL KILLING
RADIOINDUCTION
PHOSPHOTRANSFERASES
METABOLISM
BIOLOGICAL RADIATION EFFECTS
CATALYSIS
DOSE-RESPONSE RELATIONSHIPS
HAMSTERS
MUTATION FREQUENCY
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
ENZYMES
MAMMALS
RADIATION EFFECTS
RADIATIONS
RODENTS
TRANSFERASES
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)