Characterization and in vivo regulation of V sub 1 -type vasopressin receptors in the rat brain

Book · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7168273

Shewey, L M

Specific, high affinity binding sites for ({sup 3}H)-arginine{sup 8}-vasopressin (AVP) have been characterized in Long-Evans rat septal membranes. Binding displacement studies with peptide analogs of AVP indicate that this binding site is similar to the V{sub 1} (pressor)-type receptor for AVP. When added to rat brain septal slices that had been pre-labeled with ({sup 3}H)-myoinositol, AVP stimulated the accumulation of ({sup 3}H)-inositol-1-phosphate (IP{sub 1}) in the presence of lithium in a dose-dependent manner. This stimulation was completely inhibited by the specific V{sub 1} antagonists, d(CH{sub 2}){sub 5}Tyr(Me)AVP, indicating that AVP stimulates hydrolysis of inositol phospholipids in rat brain septum through an interaction with V{sub 1}-type AVP receptors. Binding studies of AVP receptors in the septum of heterozygous (HE) and homozygous, Brattleboro (BB) rats revealed an increased number of receptors with a lower affinity for AVP in the HO-BB rat when compared to the HE-BB rat. AVP-stimulated accumulation of ({sup 3}H)-IP{sub 1} was significantly greater in the septum of the HO-BB rat than in the HE-BB rat. AVP receptor binding capacity correlated with release of ({sup 3}H)-IP{sub 1} for all three groups studied.

• Research Organization: Washington Univ., Seattle, WA (USA)
• OSTI ID: 7168273
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
CHEMICAL COMPOSITION
VASOPRESSIN
AFFINITY
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL MEMBRANES
DOSE-RESPONSE RELATIONSHIPS
IN VIVO
INOSITOL
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
HORMONES
HYDROGEN COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques