In vitro metabolism of t-2 mycotoxin 1,2. Interim report

Technical Report · Mon Nov 10 00:00:00 EST 1986

OSTI ID:7162201

Thompson, W L; Pace, J G; O'Brien, J C

In vitro metabolism of T-2 mycotoxin (T-2) was studied in Vero cells, rat spleen lymphocytes, chicken embryo heart cells, rat small intestinal segments, and rat liver hepatocytes. The method used was thin-layer chromatography (TLC) of (3H)T-2 and its metabolic products, followed by radioactive scanning of the plates. Vero cells, lymphocytes, and heart cells metabolized 5 to 35% of the T-2 to HT-2 mycotoxin (HT-2) after 24-hr exposure. No other metabolites were detected with these three cell systems. Rat intestinal segments, everted onto pipets, converted T-2 into three metabolites migrating in the range between T-2 tetraol and HT-2 on the TLC plates. Hepatocytes metabolized T-2 most rapidly, as indicated by complete disappearance of the parent compound within 4 hr. In addition to the T-2 peak, four predominant peaks appeared on the plates, one of them, increasing with time at the origin, was predominantly composed of glucuronide conjugates.

• Research Organization: Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
• OSTI ID: 7162201
• Report Number(s): AD-A-192647/6/XAB
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL WARFARE AGENTS
TOXINS
METABOLITES
RADIOIMMUNOASSAY
MYCOBACTERIUM
METABOLISM
CHICKENS
EMBRYOS
FUSARIUM
HEART
LIVER
LYMPHOCYTES
RATS
SPLEEN
TOXICITY
ANIMAL CELLS
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
FOWL
FUNGI
GLANDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
ORGANS
PARASITES
PLANTS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TRACER TECHNIQUES
VERTEBRATES
WEAPONS
550900* - Pathology
550200 - Biochemistry