Early ultrastructural changes in osteocytes from the proximal tibial metaphysis of mice after the incorporation of /sup 224/Ra
Osteocytes from the proximal tibial metaphysis of mice were examined electron microscopically at various time intervals after the incorporation of 1, 1.5, or 5 ..mu..Ci/kg of body weight /sup 224/Ra. The animals were sacrificed 2 hr, 24 hr, or 5 days following administration of the short-lived bone-seeking radionuclide. Only the younger, osteoblast-like osteocytes from the undecalcified trabecular bone were studied. Compared with the osteocytes from bone tissue of control animals, about half of the cells examined in specimens from animals treated with /sup 224/Ra showed ultrastructural alterations. The chromatin in the nuclei of many osteocytes was condensed. The mitochondria showed various signs of damage such as loss of cristae, swelling, and dissociation or disruption of both limiting membranes. Additionally, giant mitochondria were found. The rough-surfaced endoplasmic reticulum was sometimes dilated and formed large cisternae. The Golgi complex was vacuolated. The pericellular spaces of lacunae containing severely damaged osteocytes were enlarged. It is thought that the early ultrastructural changes in osteocytes from the trabecular bone of mice resulted from the effects of direct irradiation upon the cells. Probably, all osteocytes which showed lesions were situated within the range of the ..cap alpha.. radiation emitted from the incorporated /sup 224/Ra which was randomly distributed in the mineralized bone matrix of the trabeculae. The mitochondria of the osteoblast-like osteocytes appeared to be the cellular organelles most sensitive to the effects of /sup 224/Ra administration.
- Research Organization:
- Gesellschaft fuer Strahlen- und Umweltforschung mbH, Munich
- OSTI ID:
- 7122239
- Journal Information:
- Radiat. Res.; (United States), Vol. 69:1
- Country of Publication:
- United States
- Language:
- English
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BONE CELLS
ELECTRON MICROSCOPY
RADIONUCLIDE KINETICS
CHROMATIN
MITOCHONDRIA
ORGANOIDS
RADIUM 224
UPTAKE
MICE
TIBIA
ALKALINE EARTH ISOTOPES
ALPHA DECAY RADIOISOTOPES
ANIMAL CELLS
ANIMALS
BODY
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-EVEN NUCLEI
HEAVY NUCLEI
ISOTOPES
MAMMALS
MICROSCOPY
NUCLEI
ORGANS
RADIOISOTOPES
RADIUM ISOTOPES
RODENTS
SKELETON
SOMATIC CELLS
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)