Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells

Journal Article · Tue Jan 09 00:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00453a031· OSTI ID:7118504

Moore, F; Riordan, J F ^[1]

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1. Harvard Medical School, Boston, MA (USA)

Angiogenin activates the phosphoinositide-specific phospholipase C (PLC) in cultured rat aortic smooth muscle cells to yield a transient (30 s) peak of 1,2-diacylglycerol (DG) and inositol trisphosphate. Within 1 min, the DG level falls below that of the control and remains so for at least 20 min. A transient increase in monoacylglycerol indicates that depletion of DG may be the consequence of hydrolysis by DG lipase. In addition to these changes in second messengers, a rapid increase in incorporating of radiolabeled tracer into cellular cholesterol esters is observed. Stimulated cholesterol ester labeling is inhibited by preincubation with either the DG lipase inhibitor RHC 80267 or the acyl coenzyme A:cholesterol acyltransferase inhibitor Sandoz 58035. Cells prelabeled with ({sup 3}H)arachidonate show a sustained increase in labeling of cholesterol esters following exposure to angiogenin. In contrast, cells prelabeled with ({sup 3}H)oleate show only a transient elevation that returns to the basal level by 5 min. This suggests initial cholesterol esterification by oleate followed by arachidonate that is released by stimulation of the PLC/DG lipase pathway.

• OSTI ID: 7118504
• Journal Information: Biochemistry; (USA), Vol. 29:1; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLESTEROL
ESTERIFICATION
LIPASES
ENZYME INDUCTION
PROTEINS
BIOCHEMISTRY
ANIMAL CELLS
AORTA
ARACHIDONIC ACID
BLOOD VESSELS
MUSCLES
OLEIC ACID
PHOSPHOLIPIDS
RATS
TRITIUM COMPOUNDS
ANIMALS
ARTERIES
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHEMISTRY
ENZYMES
ESTERASES
ESTERS
GENE REGULATION
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RODENTS
STEROIDS
STEROLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques