Autoimmunity induced by HgCl/sub 2/ in Brown-Norway rats. I. Production of monoclonal antibodies
Mercuric chloride (HgCl/sub 2/) induces in Brown-Norway rats (BN) a B cell polyclonal activation resulting in autoimmune diseases. Spleen cells from BN rats injected with HgCl/sub 2/ were fused with IR983F, a non-secreting rat myeloma cell line, in order to obtain monoclonal antibodies reacting with autoantigens or IgE-producing hybridomas. After screening for immunoglobulin-producing clones, the authors found 5% clones with anti-tissue activity, 8% with anti-TNP activity, and 41% secreting IgE. Among the anti-tissue monoclonal antibodies, one recognizes both TNP and mesangial structures of rat normal glomeruli, which could be an as yet unrecognized mechanism of nephrotoxicity. These experiments 1) confirm that HgCl/sub 2/ induces polyclonal activation, 2) show that the mercury model is of interest to obtain monoclonal IgE and various autoantibodies, and 3) suggest a new possible mechanism of antibody-mediated renal injury.
- Research Organization:
- INSERM, Paris, France
- OSTI ID:
- 7094265
- Journal Information:
- J. Immunol.; (United States), Vol. 136:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MERCURY CHLORIDES
BIOLOGICAL EFFECTS
GLOMERULI
HYBRIDOMAS
IMMUNITY
MONOCLONAL ANTIBODIES
NEPHRITIS
RATS
SPLEEN CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BODY
CHLORIDES
CHLORINE COMPOUNDS
DISEASES
HALIDES
HALOGEN COMPOUNDS
KIDNEYS
MAMMALS
MERCURY COMPOUNDS
MERCURY HALIDES
ORGANS
RODENTS
SOMATIC CELLS
UROGENITAL SYSTEM DISEASES
VERTEBRATES
550200* - Biochemistry