Pharmacokinetics of rhodanese-thiosulfate loaded murine carrier erythrocytes

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7080605

Leung, P; Yao, C C; Makary, M H; Way, J L

Since the therapeutic potential of murine erythrocytes employed in the encapsulation of rhodanese as a cyanide detoxification mechanism is dependent on a prolong circulatory lifespan, in vivo survival studies were initiated in normal adult Balb/c mice. After a single intravenous administration of the /sup 14/C-sucrose loaded carrier erythrocytes, radioactive analyses in whole blood indicated a biexponential decay. Initially, a rapid decline in /sup 14/C-sucrose radioactivity in whole blood (t/sub 1/2/ = 23 minutes) is followed by a slower decline with an apparent t/sub 1/2/ of 11 days. In contrast, when /sup 14/C-sucrose was not encapsulated, greater than 90% was eliminated from the circulation within 10 minutes. Leakage of /sup 14/C-sucrose out of the carrier erythrocytes is minimal, since less than 2% of the initial dose of encapsulated /sup 14/C-sucrose appear in plasma. When rhodanese-loaded carrier erythrocytes was employed, a similar survival curve was observed. In summary, these results suggest that carrier erythrocytes represent a viable alternative approach for the detoxification of exogenous chemical toxicants.

• Research Organization: Texas A and M Univ., College Station
• OSTI ID: 7080605
• Report Number(s): CONF-8604222-; TRN: 87-009087
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CYANIDES
DETOXIFICATION
ERYTHROCYTES
SURVIVAL TIME
THIOSULFATES
BIOCHEMICAL REACTION KINETICS
BLOOD
CARBON 14 COMPOUNDS
IN VIVO
MICE
SACCHAROSE
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
DISACCHARIDES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
REACTION KINETICS
RODENTS
SACCHARIDES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques