Linkage mapping of the human thromboxane A[sub 2] receptor (TBXA2R) to chromosome 19p13. 3 using transcribed 3[prime] untranslated DNA sequence polymorphisms

Journal Article · Mon Nov 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1457· OSTI ID:7076634

Schwengel, D A; Nouri, N; Levitt, R C; Meyers, D A ^[1]

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1. Johns Hopkins Medical Institutions, Baltimore, MD (United States)

The actions of thromboxane A[sub 2] as a prostaglandin mediator are dependent on its recently cloned and sequenced receptor. The identification and characterization of DNA polymorphisms in the thromboxane A[sub 2] receptor (TBXA2R) will advance the study of this gene as a candidate in a number of medical disorders. The authors amplified a 573-nucleotide fragment of the transcribed 3[prime] untranslated region of the TBXA2R gene using the polymerase chain reaction (PCR) and the published cDNA sequence. This region was found to contain two sequence polymorphisms within an Alu. These DNA polymorphisms were demonstrated using an efficient method of direct solid-phase sequence analysis. Three of the four expected alleles were observed in the CEPH families. TBXA2R was localized to chromosome 19 by PCR amplification in a series of monochromosomal human/rodent somatic cell hybrids. Linkage mapping places TBXA2R closest to the anonymous marker D19S120, with a maximal LOD = 19.55, at a [theta] = 0.05 in the CEPH panel of DNAs. Multipoint linkage analysis places TBXA2R between the markers D19S120 and PMS207 on the telomeric end of chromosome 19p13.3. 25 refs., 2 figs., 1 tab.

• OSTI ID: 7076634
• Journal Information: Genomics; (United States), Vol. 18:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
DNA-CLONING
HUMAN CHROMOSOME 19
GENETIC MAPPING
PROSTAGLANDINS
METABOLISM
RECEPTORS
THROMBOSIS
CARDIOVASCULAR DISEASES
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VASCULAR DISEASES
550400* - Genetics