Polymorphism in the ribosomal RNA genes in three heterothallic species of Neurospora
The authors have studied differences in molecular structures of ribosomal RNA genes (rDNAs) from different heterothallic species of Neurospora: N. crassa, N. intermedia and N. sitophila to detect any possible differences in rDNA sequences of these three species. The authors have made a new clone (named by us as pCC3400) which contains 3250 bps of the external spacer region of N. crassa wild type strain 74A rDNA sequences. This should contain both the promoter and termination regulatory sites for transcription of rRNA. Using this probe the authors have identified that about 300 bp sequences of the external spacer region present in N. crassa are apparently deleted in the species N. intermedia and N. sitophila, based on restriction patterns of 12 different enzymes. Also new restriction sites were determined as a result of the DNA sequencing of the entire ITS (internal transcribed spacer) region of the pMF2 N. crassa rDNA clone. These results were used to look at differences in the ITS regions. In addition the authors have identified a Sma 1 site within 26S rDNA which is deleted from N. intermedia but present in both N. sitophila and N. crassa. An Nru 1 site was detected in the NTES regions of N. sitophila and of N. intermedia which was not present in that position in N. crassa. All of these studies clearly establish polymorphism of Neurospora rRNA genes.
- Research Organization:
- Howard Univ., Washington, DC
- OSTI ID:
- 7065548
- Journal Information:
- Genetics; (United States), Vol. 107:3
- Country of Publication:
- United States
- Language:
- English
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Neurospora ribosomal DNA sequences are indistinguishable within cell types but distinguishable among heterothallic species
Differential gene expression in Neurospora crassa cell types: heterogeneity and multiple copies of rRNA genes. Annual progress report, July 1981-June 1982