Genomic organization and chromosomal localization of the human CD27 gene

Journal Article · Tue Dec 15 00:00:00 EST 1992 · Journal of Immunology; (United States)

OSTI ID:7064028

Loenen, W A.M.; Gravestein, L A; Beumer, S; Borst, J ^[1]; Melief, C J.M. ^[2]; Hagemeijer, A ^[3]

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1. Netherlands Cancer Inst., Amsterdam (Netherlands)
2. Academic Hospital, Leiden (Netherlands)
3. Erasmus Univ., Rotterdam (Netherlands)

CD27 is a lymphocyte-specific member of a recently identified receptor family with at least 10 members that includes the receptors for nerve growth factor and TNF, CD40, and Fas. Several members of this family play a role in cell differentiation, proliferation, and survival. Within the amino terminal ligand binding domain of these receptors, repeat motifs have been identified. These repeats contain many cysteine residues in a conserved pattern, characteristic of this family. The authors have isolated and characterized the human CD27 gene to gain insight into the evolution of this type of receptor domain. The gene was localized on chromosome 12, band 12p13. Sequence analysis showed no correlation between the intron/exon organization and the subdivision of the protein into distinct domains. Structural information for the cysteine-rich domain is contained within three exons. In addition, the splice sites in the CD27 gene are located in a different position from those in the related nerve growth factor receptor gene. However, a comparison of the splice sites within the regions encoding the respective ligand-binding domains of the CD27 and nerve growth factor receptor genes identifies the archetypal cysteine-rich building blocks, from which the members of this family may have arisen during the course of evolution. From this observation, they propose a new organization of the repeat motifs. 57 refs., 5 figs., 1 tab.

• OSTI ID: 7064028
• Journal Information: Journal of Immunology; (United States), Vol. 149:12; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
MOLECULAR STRUCTURE
LYMPHOCYTES
RECEPTORS
DNA SEQUENCING
GENETIC MAPPING
PROTEIN STRUCTURE
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAPPING
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics
550200 - Biochemistry