In vitro and in vivo properties of human/mouse chimeric monoclonal antibody specific for common acute lymphocytic leukemia antigen

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:7063271

Saga, T; Endo, K; Koizumi, M; Kawamura, Y; Watanabe, Y; Konishi, J; Ueda, R; Nishimura, Y; Yokoyama, M; Watanabe, T ^[1]

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1. Kyoto Univ. School of Medicine (Japan)

A human/mouse chimeric monoclonal antibody specific for a common acute lymphocytic leukemia antigen was efficiently obtained by ligating human heavy-chain enhancer element to the chimeric heavy- and light-chain genes. Cell binding and competitive inhibition assays of both radioiodine and indium-111- (111In) labeled chimeric antibodies demonstrated in vitro immunoreactivity identical with that of the parental murine monoclonal antibodies. The biodistribution of the radiolabeled chimeric antibody in tumor-bearing nude mice was similar to that of the parental murine antibody. Tumor accumulation of radioiodinated parental and chimeric antibodies was lower than that of {sup 111}In-labeled antibodies, probably because of dehalogenation of the radioiodinated antibodies. Indium-111-labeled chimeric antibody clearly visualized xenografted tumor. These results suggest that a human/mouse chimeric antibody can be labeled with {sup 111}In and radioiodine without the loss of its immunoreactivity, and that chimeric antibody localizes in vivo in the same way as the parental murine antibody.

• OSTI ID: 7063271
• Journal Information: Journal of Nuclear Medicine; (USA), Vol. 31:6; ISSN 0161-5505
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
MONOCLONAL ANTIBODIES
SPECIFICITY
ANTIGENS
BIOLOGICAL LOCALIZATION
CHIMERAS
EXPERIMENTAL NEOPLASMS
GENES
IMMUNE REACTIONS
IN VITRO
IN VIVO
INDIUM 111
IODINE ISOTOPES
LABELLING
LEUKEMIA
MAN
MICE
TISSUE DISTRIBUTION
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MOSAICISM
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
RODENTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550604 - Medicine- Unsealed Radionuclides in Therapy- (1980-)