Induction of sensitivity of fibroblast cultures to pituitary growth hormone by a thermostable serum factor

Journal Article · Wed May 01 00:00:00 EDT 1985 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:7058252

Bulatov, A A; Osipova, T A; Pankov, Y A; Terekhov, S M

This paper presents data to show that highly purified pituitary growth hormone (GH) preparations, themselves unable to stimulate DNA biosynthesis in cultures of adult human skin fibroblasts, acquire this ability if the cells are treated simultaneously with a factor present in a thermostable and acid-resistant fraction of rat blood serum. Activity of this factor in rat blood serum has been shown to depend on the pituitary, and to increase after hypophysectomy. Human GH, while not exhibiting activity itself, if added to the medium simultaneously with serum fraction from hypophysectomized rats, stimulated DNA biosynthesis by fibroblasts significantly. The increase in tritium-thymidine incorporation under the influence of the hormone together with the serum fraction amounted to 233%. It is important to not that serum fraction of intact rats of the same age in a concentration of 1% was unable to induce sensitivity of the fibroblasts to human GH.

• Research Organization: Institute of Experimental Endocrinology and Hormone Chemistry
• OSTI ID: 7058252
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 98:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD SERUM
BIOLOGICAL EFFECTS
DNA
BIOSYNTHESIS
FIBROBLASTS
GROWTH
RADIORECEPTOR ASSAY
STIMULATION
PITUITARY HORMONES
CELL CULTURES
MAN
RATS
SKIN
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BODY
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PRIMATES
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques