Nuclear magnetic resonance of the filamentous bacteriophage fd

Journal Article · Wed Oct 01 00:00:00 EDT 1980 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(80)84988-5· OSTI ID:7032424

Opella, S J; Cross, T A; DiVerdi, J A; Sturm, C F

The filamentous bacteriophage fd and its major coat protein are being studied by nuclear magnetic resonance (NMR) spectroscopy. /sup 31/P NMR shows that the chemical shielding tensor of the DNA phosphates of fd in solution is only slightly reduced in magnitude by motional averaging, indicating the DNA-protein interactions substantially immobilize the DNA packaged in the virus. There is no evidence of chemical interactions between the DNA backbone and the coat protein, since experiments on solid virus show the /sup 31/P resonances to have the same principal elements of its chemical shielding tensor as DNA. /sup 1/H and /sup 13/C NMR spectra of fd virus in solution indicate that the coat proteins are held rigidly in the structure except for some aliphatic side chains that undergo relatively rapid rotations. The presence of limited mobility in the viral coat proteins is substantiated by finding large quadrupole splittings in /sup 2/H NMR of deuterium labeled virions. The structure of the coat protein in a lipid environment differs significantly from that found for the assembled virus. Data from /sup 1/H and /sup 13/C NMR chemical shifts, amide proton exchange rates, and /sup 13/C relaxation measurements show that the coat protein in sodium dodecyl sulfate micelles has a native folded structure that varies from that of a typical globular protein or the coat protein in the virus by having a partially flexible backbone and some rapidly rotating aromatic rings.

• Research Organization: Univ. of Pennsylvania, Philadelphia, PA (United States)
• OSTI ID: 7032424
• Journal Information: Biophys. J.; (United States), Vol. 32:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
MORPHOLOGY
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
CARBON 13
DNA
HYDROGEN 1
LABELLED COMPOUNDS
NMR SPECTRA
PHOSPHORUS 31
ULTRASTRUCTURAL CHANGES
CARBON ISOTOPES
EVEN-ODD NUCLEI
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MORPHOLOGICAL CHANGES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
VIRUSES
550200* - Biochemistry
550700 - Microbiology
550800 - Morphology