Molecular cloning, cDNA analysis, and localization of a monomer of the N-acetylglucosamine-specific receptor of the thyroid, NAGR1, to chromosome 19p13. 3-13. 2

Journal Article · Sun May 01 00:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1219· OSTI ID:7029586

Blanck, O; Perrin, C; Mziaut, H; Miquelis, R ^[1]; Darbon, H ^[2]; Mattei, M G ^[3]

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1. Laboratoire de Biochimie, Marseille (France)
2. Laboratoire de Cristallographie et Cristallisation de Macromolecules Biologiques, Marseille (France)
3. Hopital d'enfants, Marseille (France)

The authors have proposed that the GlcNAc thyroid receptor triggers selective recycling of immature GlcNAc-bearing thyroglobulin molecules through the Golgi back to the apical membrane for further processing until maturation is achieved. This process, which is called [open quotes]receptor-mediated exocytosis,[close quotes] prevents lysosomal degradation of thyroid prohormones. In the present study, the authors report cloning of the cDNA encoding the (or one of the) monomer(s) constituting the human GlcNAc thyroid receptor. This novel gene, called NAGR1, was assigned by in situ hybridization to subbands p13.3-p13.2 of chromosome 19. Northern blot analysis showed that the mRNA encoding NAGR1 was present as a single transcript of 2.1 kb in the thyroid, but not in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The deduced amino acid sequence comprised a 51-kDa type I membrane protein with a single spanning region and a short intracytoplasmic domain. Sequence analysis showed that NAGR1 is a glycine-, tryptophan-, and methionine-rich protein with no cysteine residues or glycosylation site. No sequence homology with any known cDNA or protein was noted. The extracellular domain is composed of 420 amino acids and contains a region of 204 residues showing 15 repeats of 4 amino acids, each 1 having an acidic amino acid presumably involved in calcium coordination. The intracellular domain contained what appeared to be a tyrosine internalization signal. The usefulness of this clone in glycobiology, cell biology, and thyroid pathology studies is discussed. 53 refs., 6 figs.

• OSTI ID: 7029586
• Journal Information: Genomics; (United States), Vol. 21:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
DNA-CLONING
HUMAN CHROMOSOME 19
GENETIC MAPPING
RECEPTORS
GLUCOSAMINE
AMINES
BIOTECHNOLOGY
CARBOHYDRATES
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
GENETIC ENGINEERING
HEXOSAMINES
HEXOSES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
MEMBRANE PROTEINS
MONOSACCHARIDES
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
PROTEINS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics