Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase
Mouse alkaline phosphatase was partially purified from placenta. Data obtained by immunoblotting analysis suggested that the primary structure of this enzyme has a much greater homology to that of human and bovine liver ALPs than to the human placental isozyme. Therefore, a full-length cDNA encoding human liver-type ALP was used as a probe to isolate the mouse placental ALP cDNA. The cloned mouse cDNA is 2459 base pairs long and is composed of an open reading frame encoding a 524-amino acid polypeptide that contains a putative signal peptide of 17 amino acids. Homology at the amino acid level of the mouse placental ALP is 90% to the human liver isozyme but only 55% to the human placental counterpart. RNA blot hybridization results indicate that the mouse placental ALP is encoded by a gene identical to the gene expressed in mouse liver, kidney, and teratocarcinoma stem cells. This gene is therefore evolutionarily highly conserved in mouse and human.
- Research Organization:
- Institute for Cancer Research, Philadelphia, PA (USA)
- OSTI ID:
- 7027188
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:20
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALKALINE PHOSPHATASE
DNA SEQUENCING
PURIFICATION
GENES
BIOLOGICAL EVOLUTION
AMINO ACID SEQUENCE
DNA-CLONING
HYBRIDIZATION
MAN
MICE
PHOSPHORUS 32
PLACENTA
STEM CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
FETAL MEMBRANES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
PHOSPHATASES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques