Decreased synthesis of extracellular matrix components by chondrocytes cultured in scorbutic guinea pig serum plus vitamin C

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7025170

Oyamada, I; Bird, T A; Peterkofsky, B

The authors have previously shown that cartilage collagen (col) and proteoglycan (PG) syntheses decreased coordinately in guinea pig (GP) receiving a vitamin C deficient (C-def) diet for more than 2 weeks. The defects were related to the fasting state induced during the 3rd and 4th weeks of scurvy, rather than to the role of ascorbate in proline (pro) hydroxylation. These results and the generalized effect on col synthesis suggested the involvement of humoral factors. Sera from normal (NGPS) and scorbutic (SGPS) GP supported growth of BALB 3T3 cells under conditions where EGF plus insulin (or IGF I) were required, for up to 2 weeks after initiation of the C-def diet. Thereafter activity was lost from SGPS. The ability of NGPS and 4-week-SGPS, both with added ascorbate, to maintain normal rates of col and PG syntheses in cultured chick embryo chondrocytes was measured by incorporation of (/sup 14/C)pro into collagenase digestible protein and (/sup 35/S)sulfate into PG. Although pro hydroxylation and col secretion were normal in cells cultured with SGPS for 2 days, col and PG synthetic rates were decreased to 40-50% of rates in cells cultured in NGPS, with no change in the types of col and PG synthesized. These results provide evidence that depletion of growth factors during the fasting stage of scurvy may cause decreased synthesis of extracellular matrix components.

• Research Organization: National Cancer Institute, Bethesda, MD
• OSTI ID: 7025170
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCORBIC ACID
NUTRITIONAL DEFICIENCY
FIBROBLASTS
GROWTH
PROTEINS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
FASTING
GUINEA PIGS
SULFATES
SULFUR 35
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques