C5-derived activity is required for complement neutrophil-mediated lung injury

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7023639

Morganroth, M L; Till, G O; Ward, P A

Cobra venom factor (CVF) derived from the cobra species Naja naja (NN) is a complement activator (cleaves C3, C5 and terminal components) which causes neutrophil dependent acute lung injury and pulmonary hypertension in rats. CVF, derived from the cobra species Naja haje (NH), differs from NN-CVF in that only C3 is cleaved. The authors investigated in isolated blood perfused rat lungs if NH-CVF (n=5), a complement activator which does not generate C5 derived peptides, causes pulmonary hypertension and acute lung injury. NN-CVF (n=5) caused a transient increase in mean pulmonary artery pressure (PA) which returned toward baseline by 30 min (Peak ..delta.. PA = 17 +/- 6 mmHg). Lung injury occurred and was quantitated by the leak of /sup 125/I bovine serum albumin (/sup 125/I-BSA added 30 min post CVF) into lung parenchyma (lung/perfusate /sup 125/I-BSA counts: control = .09 +/- .01, NN-CVF = .48 +/- .11, p < .05). NH-CVF, in an amount which caused equipotent in vitro hemolysis of nonsensitized rabbit erythrocytes, did not cause a PA pressor response (3.5 +/- 1.1 mm Hg, p=NS) or acute lung injury (lung/perfusate /sup 125/I-BSA counts .11 +/- .01, p=NS). Similarly, NH-CVF did not appear to cause lung injury in intact rats (lung perfusate /sup 125/I-BSA counts: control .20 +/- .01, n=3; NH-CVF .25 +/- .05, n=3; NN-CVF .83 +/- .27, n=2). Thus, C3 cleavage alone without the generation of C5 derived peptides is an inadequate stimulus to elicit neutrophil-mediated acute lung injury.

• Research Organization: Dept. of Internal Medicine and Dept. of Pathology, Ann Arbor, MI
• OSTI ID: 7023639
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COMPLEMENT
BIOCHEMICAL REACTION KINETICS
CLEAVAGE
HYPERTENSION
PATHOGENESIS
LUNGS
INJURIES
ALBUMINS
BLOOD PRESSURE
IODINE 125
NEUTROPHILS
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CRYSTAL STRUCTURE
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MICROSTRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques