Ozone (O/sub 3/)-induced augmentation of eicosanoid metabolism in cultured epithelial cells derived from bovine trachea

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7022215

Leikauf, G D; Driscoll, K; Weideman, P; Lippmann, M

Epithelial damage and inflammation have been implicated in ozone-induced airway hyperresponsiveness. Because O/sub 3/ is relatively insoluble and highly reactive, initiating effects of this compound are likely limited to the epithelium. To examine eicosanoid metabolism following exposure to 0.1-10.0 ppm O/sub 3/, epithelial cell monolayers derived from bovine trachea were alternately exposed to O/sub 3/ and culture medium for 2h by rotating on inclined platforms. After control exposures, basal release rates determined by RIA were: PG(Prostaglandin) E/sub 2/ = 93.0(19.6), PGF/sub 2..cap alpha../=4.0(1.3), 6kPGF/sub 1..cap alpha../=4.9(0.9), Tx (Thromboxane) B/sub 2/=3.9(0.9), LT(Leukotriene)B/sub 4/=0.6(0.4)pg/cm/sup 2/ x h (mean (SE), n=6 to 22). After 0.1 ppm release of PGF/sub 2..cap alpha../ and 6kPGF/sub 1..cap alpha../ increased to 35.5(6.5) and 14.0(1.1)pg/cm/sup 2/ x h, while PGE/sub 2/ decreased to 76.5 (20.7). Thus, the F/sub 2..cap alpha..//E/sub 2/ ratio changed from 3.9(control) to 46.4% (0.1 ppm). Higher O/sub 3/ levels produced dose-dependent increases in cyclooxygenase product release with maximal rates occurring at 4.1 ppm (PGE/sub 2/ = 1170(314), PGF/sub 2..cap alpha../=26.9(8.2), 6kPGF/sub 1..cap alpha../=28.5(6.7)). Lipoxygenase products were much lower and unchanged by O/sub 3/ concentrations < 4.1 ppm. At 4.1 ppm, however, LTB/sub 4/ rates did increase to 4.3(0.9). After 10 ppm, all rates declined along with a decline in cell viability (98.8 to 93.0%). These findings suggest that environmentally relevant levels of O/sub 3/ augments cyclooxygenase and, at higher concentrations, lipoxygenase metabolism.

• Research Organization: New York Univ. Medical Center, NY
• OSTI ID: 7022215
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EICOSANOIC ACID
METABOLISM
OZONE
BIOLOGICAL EFFECTS
ARACHIDONIC ACID
CATTLE
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
OXYGENASES
PROSTAGLANDINS
TRACHEA
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
DOMESTIC ANIMALS
ENZYMES
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RESPIRATORY SYSTEM
RUMINANTS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism &amp; Toxicology