Functional somatostatin receptors on a rat pancreatic acinar cell line

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:7009531

Viguerie, N; Tahiri-Jouti, N; Esteve, J P; Clerc, P; Logsdon, C; Svoboda, M; Susini, C; Vaysse, N; Ribet, A ^[1]

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1. Institut National de la Recherche Medicale, Toulouse (France) Mount Zion Hospital and Medical Center, San Francisco, CA (USA) Universite Libre de Bruxelles, Brussels (Belgium)

Somatostatin receptors from a rat pancreatic acinar cell line, AR4-2J, were characterized biochemically, structurally, and functionally. Binding of {sup 125}I-(Tyr{sup 11})Somatostatin to AR4-2J cells was saturable, exhibiting a single class of high-affinity binding sites with a maximal binding capacity of 258 {plus minus} 20 fmol/10{sup 6} cells. Somatostatin receptor structure was analyzed by covalently cross-linking {sup 125}I-(Tyr{sup 11})somatostatin to its plasma membrane receptors. Gel electrophoresis and autoradiography of cross-linked proteins revealed a peptide containing the somatostatin receptor. Somatostatin inhibited vasoactive intestinal peptide (VIP)-stimulated adenosine 3{prime},5{prime}-cyclic monophosphate (cAMP) formation in a dose-dependent manner. The concentration of somatostatin that caused half-maximal inhibition of cAMP formation was close to the receptor affinity for somatostatin. Pertussis toxin pretreatment of AR4-2J cells prevented somatostatin inhibition of VIP-stimulated cAMP formation as well as somatostatin binding. The authors conclude that AR4-2J cells exhibit functional somatostatin receptors that retain both specificity and affinity of the pancreatic acinar cell somatostatin receptors and act via the pertussis toxin-sensitive guanine nucleotide-binding protein N{sub i} to inhibit adenylate cyclase.

• OSTI ID: 7009531
• Journal Information: American Journal of Physiology; (USA), Vol. 255:1; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MEMBRANE PROTEINS
AUTORADIOGRAPHY
RECEPTORS
CROSS-LINKING
SOMATOSTATIN
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CELL MEMBRANES
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
IODINE 125
MOLECULAR STRUCTURE
PANCREAS
RATS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550301* - Cytology- Tracer Techniques