/sup 23/Na nuclear magnetic resonance studies of ion transport in the rabbit proximal tubule

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:7002116

Avison, M J

/sup 23/Na nuclear magnetic resonance (n.m.r.) spectroscopy was used to measure intracellular sodium levels in suspensions of rabbit proximal tubules. The intracellular sodium signal was resolved by incorporating the paramagnetic shift reagent dysprosium tripolyphosphate into the suspension buffers. This shift reagent was membrane impermeant, and induced a large upfield shift of the n.m.r. signal from the extracellular sodium pool, while leaving the intracellular pool unshifted. Tubule viability and transport characteristics were unimpaired in the presence of the shift reagent provided there was no excess tripolyphosphate, and extra calcium was added to the buffer.

• Research Organization: Yale Univ., New Haven, CT (USA)
• OSTI ID: 7002116
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SODIUM COMPOUNDS
MEMBRANE TRANSPORT
BUFFERS
CHEMICAL SHIFT
NUCLEAR MAGNETIC RESONANCE
RABBITS
REAGENTS
SODIUM 23
TRACER TECHNIQUES
TUBULES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BODY
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RESONANCE
SODIUM ISOTOPES
STABLE ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques