Radioimmunoassay of buprenorphine in urine: Studies in patients and in a drug clinic

Journal Article · · Journal of Analytical Toxicology; (USA)

DOI:https://doi.org/10.1093/jat/13.2.100· OSTI ID:6999186

Hand, C W; Ryan, K E; Dutt, S K; Moore, R A ^[1]; O'Connor, J; Talbot, D ^[2]; McQuay, H J ^[3]

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1. DPC European Research Institute, Witney (England)
2. Jervis Street Hospital, Dublin (Ireland)
3. Abingdon Hospital, Oxford (England)

A radioimmunoassay kit (DPC buprenorphine double antibody) was evaluated with clinical samples and samples from a drug clinic. Urine samples were collected over a 2-day period from 5 hospital in-patients receiving sublingual buprenorphine, 400 to 2000 {mu}g/day, for the relief of chronic pain. Samples were measured before and after enzymatic hydrolysis. Urine buprenorphine concentrations were measurable at all doses studied (minimum value 5.6 ng/mL) and were greater with larger doses. The increase in concentration after hydrolysis averaged 49% and was similar for all doses studied. The authors conclude that the method has extensive cross-reactivity with glucuronides of buprenorphine and its metabolites and that samples may be analyzed without prior hydrolysis. The prevalence of buprenorphine use in 97 patients attending a drug clinic was also studied. Sixty (62%) had measurable urinary buprenorphine concentrations of 1 ng/mL or more by direct assay. The buprenorphine users were significantly younger and reported significantly greater use of opiates than nonusers.

• OSTI ID: 6999186
• Journal Information: Journal of Analytical Toxicology; (USA), Vol. 13:2; ISSN 0146-4760
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ANALGESICS
RADIOIMMUNOASSAY
ENZYMATIC HYDROLYSIS
EVALUATION
PATIENTS
URINE
BIOASSAY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL REACTIONS
DECOMPOSITION
DIAGNOSTIC TECHNIQUES
DRUGS
HYDROLYSIS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LYSIS
MATERIALS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SOLVOLYSIS
TRACER TECHNIQUES
WASTES
550601* - Medicine- Unsealed Radionuclides in Diagnostics