Detection of T-2 mycotoxin metabolites in urines of exposed rats. Comparison of a potentially fieldable kit with a laboratory assay. Interim report

Technical Report · Wed Mar 09 00:00:00 EST 1988

OSTI ID:6995379

Hewetson, J F; Wannemacher, R W; Hawley, R J

Rapid methods to detect toxin exposure have been a concern of the Army since the reported use of T-2 mycotoxin as a biological warfare agent in Southeast Asia and Afghanistan. T-2 toxin was included in an exploratory development program of rapid identification systems for biological agents sponsored by the United States Army Medical Materiel Development Activity. Reported here is evidence of T-2W exposure in urines collected up to 2 weeks after rats were exposed to a sublethal dose of T-2 toxin. A laboratory radioimmunoassay (RIA) using polyclonal antibody was used to assay the urines for HT-2 or T-2 tetraol. The sensitivity of the RIA for HT-2 was 5 ng/ml and 50 ng/ml for T-2 tetraol. Some of the urines were assayed in parallel with a potentially fieldable enzyme-linked immunoassay (ELISA) developed for T-2 with a monoclonal antibody that cross reacts with HT-2.

• Research Organization: Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (USA)
• OSTI ID: 6995379
• Report Number(s): AD-A-192649/2/XAB
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL WARFARE AGENTS
METABOLITES
RADIOIMMUNOASSAY
TOXINS
URINE
ANTIBODIES
DETECTION
ENZYMES
FUSARIUM
IMMUNOASSAY
RATS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
FUNGI
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
PARASITES
PLANTS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
TOXIC MATERIALS
TRACER TECHNIQUES
VERTEBRATES
WASTES
WEAPONS
550900* - Pathology
550200 - Biochemistry