Evidence for an involvement of thymidine kinase in the excision repair of ultraviolet-irradiated herpes simplex virus in human cells

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850150104· OSTI ID:6992566

Intine, R V; Rainbow, A J ^[1]

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1. McMaster Univ., Hamilton, Ontario (Canada)

A wild-type strain of herpes simplex virus type 1 (HSV-1:KOS) encoding a functional thymidine kinase (tk+) and a tk- mutant strain (HSV-1:PTK3B) were used to study the role of the viral tk in the repair of UV-irradiated HSV-1 in human cells. UV survival of HSV-1:PTK3B was substantially reduced compared with that of HSV-1:KOS when infecting normal human cells. In contrast, the UV survival of HSV-1:PTK3B was similar to that of HSV-1:KOS when infecting excision repair-deficient cells from a xeroderma pigmentosum patient from complementation group A. These results suggest that the repair of UV-irradiated HSV-1 in human cells depends, in part at least, on expression of the viral tk and that the repair process influenced by tk activity is excision repair or a process dependent on excision repair.

• OSTI ID: 6992566
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Vol. 15:1; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PHOSPHOTRANSFERASES
GENES
XP CELLS
DNA REPAIR
HERPES SIMPLEX
MAN
MUTATIONS
THYMIDINE
ULTRAVIOLET RADIATION
VIRUSES
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
ENZYMES
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
MAMMALS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SKIN DISEASES
TRANSFERASES
VERTEBRATES
VIRAL DISEASES
560130* - Radiation Effects on Microorganisms