Intestinal zinc and carboxypeptidase activity (CP) in rats fed test meals containing various dietary proteins

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6989117

Schneeman, B O; Berger, J

Intestinal infusion of protein stimulates pancreatic zinc, CPA and CPB secretion. Soy protein isolate (SPI) stimulates a greater rate of pancreatic zinc secretion than casein (C). The objective of the present study was to determine the levels of zinc, CPA and CPB in the intestinal contents after consuming test meals which contained either C, SPI, or egg white (EW) as the protein source. Five grams of diet was offered to fasted rats during a 20 minute period. One group was killed prior to receiving a test diet and served as a 0 time group. Rats were killed at 1, 2.5, or 5 hours after initiating the food consumption period. Intestinal contents were removed and analyzed for zinc and CPA and CPB activity. At 1 h CPA and CPB activity in the intestinal contents was significantly higher in the EW and SPI group than in the C group. At 2.5 h activity remained significantly higher in the EW group than in the C group, by 5 h activity had returned to fasting levels in all groups. Total zinc in the intestinal contents was significantly higher in the SPI group than in the C or EW group at 1 and 2.5 h after consuming the test meal. Intestinal zinc levels only increased marginally from fasting levels at these times in the groups consuming C or EW. The high level of intestinal zinc in rats fed SPI suggests that the secretion and turnover of endogenous zinc could affect its bioavailability.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6989117
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBOXYPEPTIDASES
ENZYME ACTIVITY
ZINC
BIOLOGICAL EFFECTS
METABOLISM
BIOLOGICAL AVAILABILITY
DIET
INTESTINAL ABSORPTION
PROTEINS
RATS
ABSORPTION
ANIMALS
ELEMENTS
ENZYMES
HYDROLASES
MAMMALS
METALS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
RODENTS
UPTAKE
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology