In vitro comparisons of SENCAR and BALB/c primary epidermal cells
Grafting experiments show that the enhanced sensitivity of the SENCAR mouse to skin carcinogenesis by initiation and promotion is a property of the skin itself, suggesting the usefulness of in vitro studies to elucidate the mechanism. Such studies have indicated that cultured epidermal cells of SENCAR mice and the resistant BALB/c strain are remarkably similar in a variety of respects. Primary epidermal cells cultured in the presence of various concentrations of 12-O-tetradecanoylphorbol-13-acetate (TPA), retinoic acid, epidermal growth factor (EGF), hydrocortisone, or fluocinolone acetonide failed to reveal differences in growth between BALB/c and SENCAR cells. Cells from these animals bound comparable amounts of EGF with similar kinetics, and the modulation of this binding by TPA and retinoic acid was indistinguishable between strains. Spontaneous expression of infectious, endogenous xenotropic type C RNA virus at very low levels could be demonstrated in primary BALB/c epidermal cells and both BALB/c and SENCAR epidermal lines resistant to Ca/sup 2 +/-induced terminal differentiation. The number of foci of initiated cells after exposure to carcinogens in vivo or in vitro did not differ significantly between SENCAR and BALB/c, suggesting that SENCAR sensitivity is primarily to promotion. However, there are qualitative differences between SENCAR and BALB/c foci. The appearance of foci of cells resistant to terminal differentiation in untreated SENCAR cultures supports the evidence from in vivo studies for the existence of a constitutively initiated cell population in SENCAR mouse skin.
- Research Organization:
- National Institutes of Health (NIH), Bethesda, MD (United States)
- OSTI ID:
- 6985013
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 68
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOGENESIS
BIOLOGICAL MODELS
EPIDERMIS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
DNA REPAIR
IN VITRO
MICE
PHORBOL ESTERS
RETINOIC ACID
SKIN
SPECIES DIVERSITY
TRITIUM COMPOUNDS
TUMOR PROMOTERS
X RADIATION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBOXYLIC ACID ESTERS
CARCINOGENS
ELECTROMAGNETIC RADIATION
EPITHELIUM
ESTERS
IONIZING RADIATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROMOTERS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RODENTS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture