TSPY-related sequences represent a microheterogeneous gene family organized as constitutive elements in DYZ5 tandem repeat units on the human Y chromosome

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1393· OSTI ID:6975858

Manz, E; Schnieders, F; Brechlin, A M; Schmidtke, J ^[1]

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1. Medizinische Hochschule Hannover, Hannover (Germany)

TSPY (testis-specific protein, Y-encoded) is encoded by members of a Y-chromosome-specific sequence family. The authors show here that TSPY elements are part of the DYZ5 repeat unit. They have established a cosmid library of Y-chromosomal DNA derived from the hybrid cell line 3E7 and isolated eight cosmids representing 15 TSPY elements. Interindividual variability with respect to TSPY copy number was observed. One cosmid clone was investigated in detail by subcloning and sequencing. Sequence microheterogeneity was identified among both transcribed and nontranscribed TSPY elements. Transcript variability is not restricted to single basepair exchanges. They present data for the existence of at least three different sized TSPY transcripts, caused by different patterns of splicing. 13 refs., 5 figs.

• OSTI ID: 6975858
• Journal Information: Genomics; (United States), Vol. 17:3; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COSMIDS
DNA SEQUENCING
DNA-CLONING
HUMAN Y CHROMOSOME
GENETIC MAPPING
TESTES
GENES
RNA PROCESSING
BODY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
GONADS
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HYBRIDIZATION
MALE GENITALS
MAPPING
ORGANS
STRUCTURAL CHEMICAL ANALYSIS
Y CHROMOSOME
550400* - Genetics