cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences

Journal Article · Fri Oct 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1432· OSTI ID:6975322

Andres, D A; Wenzlau, J M; Brown, M S; Goldstein, J L ^[1]; Milatovich, A; Oezcelik, T; Francke, U ^[2]

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1. Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
2. Stanford Univ. Medical Center, CA (United States)

The CAAX farnesyltransferase is a heterodimeric enzyme that attaches a farnesyl group to a single cysteine in several cellular proteins. Substrates include the p21[sup ras] proteins, nuclear lamins, and several retinal proteins, all of which end with a [open quotes]CAAX box,[close quotes] where C is cysteine, A is an aliphatic amino acid, and X is methionine or serine. Full-length cDNAs for the [alpha] and [beta] subunits of the rat farnesyltransferase have been cloned, and both have been shown to be essential for catalytic activity. Here, the authors have used the rat cDNAs to clone cDNAs for the human [alpha] and [beta] subunits. Comparison of the human and rat amino acid sequences revealed a remarkable degree of conservation (93% identity for the [alpha] subunit and 96% identity for the [beta] subunit). The functional genes for the [alpha] and [beta] subunits of human farnesyltransferase (gene symbols, FNTA and FNTB) were localized to human chromosome bands 8p22-q11 and 14q23-q24, respectively, by Southern blot hybridization and PCR analyses of panels of human X Chinese hamster somatic cell hybrid lines and by fluorescence chromosomal in situ hybridization. They also found several related farnesyltransferase genes. FNTAL1 was assigned to 11q13.4-q14.1, FNTAL2 to chromosome 13, and FNTBL1 to chromosome 9. 35 refs., 3 figs., 2 tabs.

• OSTI ID: 6975322
• Journal Information: Genomics; (United States), Vol. 18:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA-CLONING
HUMAN CHROMOSOME 13
GENETIC MAPPING
HUMAN CHROMOSOME 14
HUMAN CHROMOSOME 8
HUMAN CHROMOSOME 9
TRANSFERASES
DNA SEQUENCING
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
ENZYMES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics