Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell carboxypeptidase A and rat pancreatic carboxypeptidases

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.23.9480· OSTI ID:6970212

Reynolds, D S; Gurley, D S; Stevens, R L; Austen, K F; Serafin, W E ^[1]; Sugarbaker, D J ^[2]

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1. Harvard Medical School, Cambridge, MA (USA) Brigham and Women's Hospital, Boston, MA (USA)
2. Harvard Medical School, Cambridge, MA (USA)

Human skin and lung mast cells and rodent peritoneal cells contain a carboxypeptidase in their secretory granules. The authors have screened human lung cDNA libraries with a mouse mast cell carboxypeptidase A (MC-CPA) cDNA probe to isolate a near-full-length cDNA that encodes human MC-CPA. The 5{prime} end of the human MC-CPA transcript was defined by direct mRNA sequencing and by isolation and partial sequencing of the human MC-CPA gene. Human MC-CPA is predicted to be translated as a 417 amino acid preproenzyme which includes a 15 amino acid signal peptide and a 94-amino acid activation peptide. The mature human MC-CPA enzyme has a predicted size of 36.1 kDa, a net positive charge of 16 at neutral pH, and 86% amino acid sequence identity with mouse MC-CPA. DNA blot analyses showed that human MC-CPA mRNA is transcribed from a single locus in the human genome. Comparison of the human MC-CPA with mouse MC-CPA and with three rat pancreatic carboxypeptidases shows that these enzymes are encoded by distinct but homologous genes.

• OSTI ID: 6970212
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:23; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBOXYPEPTIDASES
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA-CLONING
DNA SEQUENCING
GENES
IMMUNOLOGY
LUNGS
MAN
MAST CELLS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
PANCREAS
PHOSPHORUS 32
RATS
SKIN
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
ENDOCRINE GLANDS
ENZYMES
GLANDS
HYBRIDIZATION
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RESPIRATORY SYSTEM
RNA
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques