Differential changes in atrial natriuretic peptide and vasopressin receptor bindings in kidney of spontaneously hypertensive rat

Journal Article · Mon Jan 19 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90337-7· OSTI ID:6937672

Ogura, T; Mitsui, T; Yamamoto, I; Katayama, E; Ota, Z; Ogawa, N

To elucidate the role of atrial natriuretic peptide (ANP) and vasopressin (VP) in a hypertensive state, ANP and VP receptor bindings in spontaneously hypertensive rat (SHR) kidney were analyzed using the radiolabeled receptor assay (RRA) technique. Systolic blood pressure of SHR aged 12 weeks was statistically higher than that of age-matched Wistar Kyoto (WKY) rats. Maximum binding capacity (Bmax) of (/sup 125/I)-ANP binding to the SHR kidney membrane preparations was statistically lower than that of WKY rats, but dissociation constant (Kd) was not significantly different. On the other hand, Bmax of (/sup 3/H)-VP binding to the SHR kidney membrane preparations was statistically higher than that of WKY rats, but Kd were similar. Since the physiological action of ANP is natriuresis and VP is the most important antidiuretic hormone in mammalia, these opposite changes of ANP and VP receptor bindings in SHR kidney suggested that these peptides may play an important role in the pathophysiology of the hypertensive state, although it has not been confirmed as yet.

• Research Organization: Okayama Univ. Medical School, Japan
• OSTI ID: 6937672
• Journal Information: Life Sci.; (United States), Vol. 40:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYPERTENSION
ETIOLOGY
PEPTIDE HORMONES
BIOCHEMICAL REACTION KINETICS
RADIORECEPTOR ASSAY
IODINE 125
KIDNEYS
LABELLED COMPOUNDS
PHYSIOLOGY
RATS
RECEPTORS
TRITIUM COMPOUNDS
VASOPRESSIN
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
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