Enhanced lysis of herpes simplex virus type 1-infected mouse cell lines by NC and NK effectors
Spontaneously cytotoxic murine lymphocytes lysed certain cell types infected by herpes simplex virus type 1 (HSV-1) better than uninfected cells. Although HSV-1 adsorbed to the surface of all the target cells, those in which the virus replicated more efficiently were lysed to a greater extent. As targets, the authors used cell lines that, when uninfected, were spontaneously lysed by NK cells (YAC-1) or by NC cells (WEHI-164). They also used a fibroblastoid cell line (M50) and a monocytic tumor line (PU51R), which were not spontaneously killed. NK cells lysed HSV-1-infected YAC cells better than uninfected cells, and an NC-like activity selectively lysed HSV-1-infected WEHI cells. These findings were consistent with the results of experiments performed to define the role of interferon in induction of virus-augmented cytolysis. Increased lysis of YAC-HSV and PU51R-HSV was entirely due to interferon activation and was completely abolished by performing the /sup 51/Cr-release assay in the presence of anti-interferon serum. The data show that HSV-1 infection of NK/NC targets induces increased cytotoxity, but the effector cell responsible for lysis is determined by the uninfected target, or by an interaction between the virus and target cell, rather than by a viral determinant alone.
- Research Organization:
- Sloan-Kettering Institute for Cancer Research, New York, NY
- OSTI ID:
- 6936195
- Journal Information:
- J. Immunol.; (United States), Vol. 136:9
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
CELL KILLING
ANTIGEN-ANTIBODY REACTIONS
CELL CULTURES
CELL WALL
CHROMIUM 51
FIBROBLASTS
HERPES SIMPLEX
INTERFERON
LABELLED COMPOUNDS
LYSIS
MICE
RADIO-RELEASE ANALYSIS
TUMOR CELLS
VIRUSES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MICROORGANISMS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
RODENTS
SKIN DISEASES
SOMATIC CELLS
VERTEBRATES
VIRAL DISEASES
550300* - Cytology