Quantification of the local glucose utilization and local blood flow in the heart of the awake rat using the /sup 14/C-2-deoxyglucose and /sup 14/C-iodoantipyrine methods
Local cardiac glucose utilization (LCarGU) was quantified in the rat heart according to the Sokoloff model and local cardiac blood flow (LCarBF) according to the /sup 14/C-iodoantipyrine method. For quantitative autoradiography calibration curves for heart slices were performed. They differed from the brain calibration curves by 8%. The lumped constant was 0.377 in isolated working hearts. LCarGU and LCarBF could then be quantified in awake rats. At different locations mean LCarGU of different hearts varied from 85 to 200 ..mu..moles/100g/min and mean LCarBF from 390 to 831 ml/100g/min. The ratio subendocardial/subepicardial glucose utilization or blood flow was not systematically different from 1. The results indicate that glucose can be an important fuel in the heart of the awake rat, although its contribution to overall metabolism varies from animal to animal.
- Research Organization:
- Univ. of Bonn, West Germany
- OSTI ID:
- 6935070
- Report Number(s):
- CONF-8604222-; TRN: 87-008699
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOSE
METABOLISM
HEART
BLOOD FLOW
ANTIPYRINE
AUTORADIOGRAPHY
BIOLOGICAL VARIABILITY
CALIBRATION
CARBON 14 COMPOUNDS
RATS
ALDEHYDES
ANIMALS
ANTIPYRETICS
AZOLES
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
HETEROCYCLIC COMPOUNDS
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
PYRAZOLINES
RODENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques