Application of pulsed-gradient /sup 31/P NMR on frog muscle to measure the diffusion rates of phosphorus compounds in cells

Journal Article · Sat May 01 00:00:00 EDT 1982 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(82)84549-9· OSTI ID:6933444

Yoshizaki, K; Seo, Y; Nishikawa, H; Morimoto, T

Pulsed-gradient /sup 31/P NMR was used to measure the diffusion rates of phosphorus compounds in aqueous solution and in living muscles. The diffusion rates of creatine phosphate and inorganic phosphate in intact frog muscle cells were reduced by a factor of approx. 2 from those in aqueous solution, which suggests that the apparent intracellular viscosity is approx. 2 times larger than in aqueous solution.

• Research Organization: Kyoto Prefectural Univ. of Medicine, Japan
• OSTI ID: 6933444
• Journal Information: Biophys. J.; (United States), Vol. 38:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHORUS 31
NUCLEAR MAGNETIC RESONANCE
PHOSPHORUS COMPOUNDS
BIOCHEMICAL REACTION KINETICS
DIFFUSION
NMR SPECTRA
AQUEOUS SOLUTIONS
FROGS
MUSCLES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
DISPERSIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MIXTURES
NUCLEI
ODD-EVEN NUCLEI
PHOSPHORUS ISOTOPES
REACTION KINETICS
RESONANCE
SOLUTIONS
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques