Desensitization of animals to the inflammatory effects of ultraviolet radiation is mediated through mechanisms which are distinct from those responsible for endotoxin tolerance
Desensitization to the inflammatory properties of either UVR or LPS appears to be controlled at the site of interaction between the tissues capable of producing epidermal-derived thymocyte-activating factor (ETAF)/IL 1 (epidermal keratinocytes or reticuloendothelial cells, respectively) and the exogenous inflammatory stimulus. Peritoneal macrophages obtained from LPS-desensitized mice were found to have a markedly reduced capacity to secrete ETAF/IL 1 in vitro when compared to peritoneal exudate cells (PEC) obtained from normal mice. In parallel with this decreased secretory potential by PEC was the appearance of membrane-associated IL 1 was not found to be present on PEC obtained from normal mice. Keratinocytes obtained from the skin of normal mice or keratinocytes isolated from the irradiated skin site of UVR-desensitized mice were both found to secrete high levels of ETAF/IL 1 constitutively in vitro. Furthermore, both sources of keratinocytes also expressed membrane-associated forms of ETAF/IL 1 constitutively. Therefore, unlike LPS desensitization, the phenomenon of UVR desensitization does not appear to induce changes in the ability of keratinocytes to secrete soluble forms or to express membrane forms of ETAF/IL 1. UVR desensitization may be a result of the inability of ETAF/IL 1 generated within the skin to reach the various IL 1 responsive target cells throughout the body, or may result from the impaired ability of UVR to stimulate ETAF/IL 1 production due to changes in the structure of the skin of chronically UVR-exposed animals.
- Research Organization:
- Univ. of Utah School of Medicine, Salt Lake City
- OSTI ID:
- 6927693
- Journal Information:
- J. Immunol.; (United States), Vol. 136:8
- Country of Publication:
- United States
- Language:
- English
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LIPOPOLYSACCHARIDES
INFLAMMATION
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
CELL MEMBRANES
EPIDERMIS
MICE
SEROUS MEMBRANES
THYMOCYTES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
EPITHELIUM
LIPIDS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
POLYSACCHARIDES
RADIATIONS
RODENTS
SACCHARIDES
SKIN
SOMATIC CELLS
SYMPTOMS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals