Localization of the gene for DNA polymerase [epsilon] (POLE) to human chromosome 12q24. 3 and rat chromosome 12 by somatic cell hybrid panels and fluorescence in situ hybridization

Journal Article · Tue Mar 15 00:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1156· OSTI ID:6902843

Szpirer, J; Riviere, M; Szpirer, C ^[1]; Pedeutour, F; Turc-Carel, C ^[2]; Kesti, T; Syvaeoja, J E ^[3]

---

1. Universite Libre de Bruxelles, Rhode-St-Genese (Belgium)
2. Universite de Nice-Sophia Antipolis (France)
3. Univ. of Oulu, Linnanmaa (Finland)

DNA polymerase [epsilon] [DNA pol [epsilon] (EC 2.7.7.7)] is one of the four nuclear DNA polymerases in eukaryotic cells. The mammalian enzyme is involved in DNA repair and possibly also in replication of chromosomal DNA. The gene encoding pol [epsilon] (POLE) was assigned to human and rat chromosomes 12 by Southern blot analysis of genomic dNA from mouse-human and mouse-rat somatic cell hybrid panels using human cDNA probe. The human gene was then regionally localized to band 12q24.3 by fluorescence in situ hybridization of metaphase spreads of chromosomes from human lymphocytes using genomic DNA probe. POLE is closely linked to HNF1A, a gene encoding a liver-enriched transcription factor, HNF1[alpha]. The two genes thus define a new synteny group retained on human and rat chromosomes 12. Another gene mapping to the same or close-by region as human POLE is a gene for the inherited disorder tuberous sclerosis 3, TSC3. 19 refs., 3 figs., 1 tab.

• OSTI ID: 6902843
• Journal Information: Genomics; (United States), Vol. 20:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
GENES
HUMAN CHROMOSOME 12
GENETIC MAPPING
DNA HYBRIDIZATION
DNA-CLONING
CHROMOSOMES
CLONING
ENZYMES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
TRANSFERASES
550400* - Genetics