Combined autoradiography and immunofluorescence for estimation of single cell activity by ammonium-oxidizing bacteria

Journal Article · Thu Mar 01 00:00:00 EST 1984 · Limnol. Oceanogr.; (United States)

DOI:https://doi.org/10.4319/lo.1984.29.2.0402· OSTI ID:6896196

Ward, B B

Immunofluorescence and /sup 14/CO/sub 2/ autoradiography were used for simultaneously enumerating and assaying the autotrophic activity of ammonium-oxidizing bacteria in seawater. Relative activity (/sup 14/CO/sub 2/ assimilation as measured by autoradiography) and abundance were measured in simulated in situ incubations at seven stations in the primary NO/sub 2//sup -/ maximum region of the Northeast Pacific Ocean. More than 10/sup 4/ cells-liter/sup -1/ were present; relative activity often showed a peak near the surface and an increase in the NO/sub 2//sup -/ max region below the photic zone. The method permits assessment of individual cell activity; most cells at all depths were active in CO/sub 2/ assimilation, usually at low and quite variable levels. Relative activity was positively correlated with the abundance of ammonium-oxidizing bacteria, temperature, total dark CO/sub 2/ assimilation and phenopigment concentration.

• Research Organization: Univ. of Washington, Seattle
• DOE Contract Number: AT06-76EV75026
• OSTI ID: 6896196
• Journal Information: Limnol. Oceanogr.; (United States), Vol. 29:2
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
AMMONIUM COMPOUNDS
OXIDATION
BACTERIA
AUTORADIOGRAPHY
FLUORESCENCE
METABOLISM
CARBON 14 COMPOUNDS
CARBON DIOXIDE
NITRITES
PACIFIC OCEAN
SEAWATER
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
HYDROGEN COMPOUNDS
LABELLED COMPOUNDS
LUMINESCENCE
MICROORGANISMS
NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SEAS
SURFACE WATERS
WATER
520101* - Environment
Aquatic- Basic Studies- Radiometric Techniques- (-1989)
550701 - Microbiology- Tracer Techniques