Geobacter sulfurreducens sp. nov. , a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism
- Univ. of Oklahoma, Norman, OK (United States)
- Duquesne Univ., Pittsburgh, PA (United States)
- Geological Survey, Reston, VA (United States)
A dissimilatory metal- and sulfur-reducing microorganism was isolated from surface sediments of a hydrocarbon-contaminated ditch in Norman, Oklahoma. The isolate, which was designated strain PCA, was an obligately anaerobic, nonfermentative, nonmotile, gram-negative rod. PCA grew in a defined medium with acetate as an electron donor and ferric PP[sub i], ferric oxyhydroxide, ferric citrate, elemental sulfur, CO(III)-EDTA, fumarate, or malate as the sole electron acceptor. PCA also coupled the oxidation of hydrogen to the reduction of Fe(III) but did not reduce Fe(III) with sulfur, glucose, lactate, fumarate, propionate, butyrate, isobutryate, isovalerate, succinate, yeast extract, phenol, benzoate, ethanol, propanol, or butanol as an electron donor. PCA did not reduce oxygen, Mn(IV), U(VI), nitrate, sulfate, sulfite, or thiosulfate with acetate as the electron donor. Cell suspensions of PCA exhibited dithionite-reduced minus air-oxidized difference spectra which were characteristic of c-type cytochromes. Phylogenetic analysis of the 16S rRNA sequence placed PCA in the delta subgroup of the proteobacteria. Its closest known relative is Geobacter metallireducens. The ability to utilize either hydrogen or acetate as the sole electron donor for Fe(III) reduction makes strain PCA a unique addition to the relatively small group of respiratory metal-reducing microorganisms available in pure culture. A new species name, Geobacter sulfurreducens, is proposed. 46 refs., 8 figs., 1 tab.
- OSTI ID:
- 6872758
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 60:10; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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