Toluene degradation by Pseudomonas putida F1: genetic organization of the tod operon

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6865285

Zylstra, G J; McCombie, W R; Gibson, D T; Finette, B A

Pseudomonas putida PpF1 degrades toluene through cis-toluene dihydrodiol to 3-methylcatechol. The latter compound is metabolized through the well-established meta pathway for catechol degradation. The first four steps in the pathway involve the sequential action of toluene dioxygenase (todABC1C2), cis-toluene, dihydrodiol dehydrogenase (todD), 3-methylcatechol 2,3-dioxygenase (todE), and 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase (todF). The genes for these enzymes form part of the tod operon which is responsible for the degradation of toluene by this organism. A combination of transposon mutagenesis of the PpF1 chromosome, was well as the analysis of cloned chromosomal fragments, was used to determine the physical order of the genes in the tod operon. The genes were determined to be transcribed in the order todF, todC1, todC2, todB, todA, todD, todE.

• Research Organization: Univ. of Texas, Austin (USA)
• OSTI ID: 6865285
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 54:6
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
HYDROLASES
GENES
OXIDOREDUCTASES
PSEUDOMONAS
GENE REGULATION
TOLUENE
BIODEGRADATION
BIOLOGICAL PATHWAYS
ENZYME ACTIVITY
GENE OPERONS
GENETIC MAPPING
MUTAGENESIS
TRANSCRIPTION
ALKYLATED AROMATICS
AROMATICS
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
HYDROCARBONS
MAPPING
MICROORGANISMS
ORGANIC COMPOUNDS
510200* - Environment
Terrestrial- Chemicals Monitoring & Transport- (-1989)
550400 - Genetics